- All reagents in the kit should be stored according to the manual instructions.
- Before beginning the experiment, all components should be brought to room temperature.
- To ensure the uniformity and accuracy of each reagent, mix all components before use.
- Keep the temperature stable at room temperature throughout the entire experiment.
- The pH should be maintained between 7.2-7.4 for the entire duration of the experiment.
- Ensure all concentrated reagents are appropriately diluted prior to beginning the experiment.
- Washing technique should be kept consistent throughout the experiment and done according to the instructions in the manual.
- Avoid creating bubbles in solutions to ensure uniformity of all reactions.
- Take the reading of the microtiter plate 2 minutes after adding the stop solution.